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. Author manuscript; available in PMC: 2009 Jun 22.
Published in final edited form as: J Steroid Biochem Mol Biol. 2008 Jan 19;109(1-2):150–157. doi: 10.1016/j.jsbmb.2008.01.002

Figure 6. GR phospho-isoform recruitment to the gilz regulatory region.

Figure 6

A) Schematic depiction of the gilz regulatory region. The GR binding sites (GREs) are shown as gray boxes. The small vertical arrows show other transcription factor binding sites. The horizontal arrows depict the primers pairs used to amplify the regions encompassing GREs. B–C) Phospho-GR recruitment to gilz over 180 min. U2OS-hGR cells were treated with vehicle or Dex for the times indicated and ChIP were performed with antibodies against total GR, P-S211, P-S226 and GR binding sites were amplified by PCR using gilz-specific primer pairs and the PCR products resolved, stained and quantified as in Figure 3.