Figure 4.

Relationship between reelin and migrating preganglionic neurons. (a) E11.5. CR-50 immunostaining shows that reelin is widely distributed in the newly formed ventral spinal cord. The only regions that are devoid of reelin are the neuroepithelium and the ventrolateral spinal cord, where preganglionic and somatic motor neurons are located (arrows). (b) E12. Retrograde dextran amine labeling of preganglionic neurons (green, arrows) combined with immunostaining of reelin (red) shows that reelin is present adjacent to migrating preganglionic neurons in the ventral spinal cord. (c) E12.5. Reelin is located more dorsally in the spinal cord. Again, the area devoid of reelin is where preganglionic neurons are located (arrows). (d) E14. Double immunostaining with nitric oxide synthase immunostaining for preganglionic neurons and CR-50 shows that reelin (red) is located more dorsally but is still adjacent to the preganglionic neurons (green, arrows) and separates them from regions of the central canal.