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A combined treatment of docetaxel and d-limonene increased p21, p53, Bad, cytochrome c and decreased Bcl-xL protein levels and cleavage of caspase-9, caspase-3 and PARP during 0-48 h. Immunoblotting using cytosolic fraction (for cytochrome c) and cell lysates (for all other proteins) from DU-145 cells treated with 1.9 nM docetaxel together with 0.2 mM d-limonene for the indicated time periods. The blots were stripped and reprobed with anti-actin antibody to ensure equal loading.
