The effect of different media supplements on responses to bioactive glass. A: Alkaline phosphatase activity per μg DNA for one human sample (H2), mean and S.D. grown in 4 media conditions, Asc= ascorbate alone, Asc+Dex = ascorbate plus dexamethasone, Asc+BMP-2 = ascorbate plus BMP-2 and Asc+Dex+BMP-2 = all three supplements. There are no media conditions in which alkaline phosphatase activity is higher on bioactive glass than in tissue culture plastic (n=2). B: Alkaline phosphatase activity per unit of MTS, mean and S.D. in serum free conditions for 3 different patients. Alkaline phosphatase activity is much higher in BMP-2 treated samples in serum free medium compared with controls, the effect of bioactive glass is variable, alkaline phosphatase activity is higher in one patient (H4), n=2.