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Application of the 3D model of the oral mucosa in the study of host–pathogen interactions. The organotypic cultures were infected with C. albicans strain SC5314 for (a) 8 h, (b) 14 h or (c) 24 h and tissue damage was assessed by measuring lactate dehydrogenase release into the subnatants. (b) Soft tissue biofilm forming over the epithelial layer (arrow). (c) Fungal invasion (arrows) ofthe tissueswas consistent with tissue damage. (×100 magnification.)
