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. Author manuscript; available in PMC: 2010 Jun 10.
Published in final edited form as: Structure. 2009 Jun 10;17(6):843–856. doi: 10.1016/j.str.2009.04.007

Figure 2. Small angle X-ray scattering analysis of ALIXBro1-V.

Figure 2

(A) Experimental scattering intensity patterns obtained for monomeric (dark grey) and dimeric (light grey) ALIXBro1-V are shown as a function of resolution and after averaging and subtraction of solvent scattering. The scattering intensity patterns calculated from the ALIXBro1-V monomer and dimer SAXS model with the lowest χ value are shown as black lines.

(B) P(r) function of both monomeric (dark grey) and dimeric (light grey) ALIXBro1-V (both curves have been adjusted to the same height). The ab initio model envelope of monomeric ALIXBro1-V is shown together with the manually docked ALIXBro1-V structure (Fisher et al., 2007) as inset.

(C) Ab initio modeling of dimeric ALIXBro1-V reveals a crescent shape model spanning ~ 270 Å; two orientations of the bead model including the molecular envelope are shown.