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. Author manuscript; available in PMC: 2010 Jun 10.
Published in final edited form as: Structure. 2009 Jun 10;17(6):843–856. doi: 10.1016/j.str.2009.04.007

Figure 6. ALIXBro1-V dimerizes in vivo.

Figure 6

ALIXBro1-V was expressed using a split YFP expression system. (A) Co-expression of both ALIXBro1-V YFP fusion protein halves in HEK293 cells resulted in complementation (right panel). ALIXBro1-V expression thus induces ALIXBro1-V dimerization in vivo.

(B) Expression of CHMP4BΔC-ALIX revealed inclusions partly localized along the plasma membrane (left panel).

(C) Co-expression of ALIXBro1-V YFP fusion constructs and CHMP4BΔC-ALIX; red fluorescence CHMP4BΔC-ALIX (left panel), green fluorescence dimeric ALIX (middle panel) and the lower panel show the co-localization: CHMP4BΔC-ALIX recruits ALIXBro1-V dimers in vivo into large inclusions. Control staining with DAPI is shown in all panels.