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. Author manuscript; available in PMC: 2010 Jun 10.
Published in final edited form as: Structure. 2009 Jun 10;17(6):843–856. doi: 10.1016/j.str.2009.04.007

Figure 7. ALIX dimerization is important for HIV-1 budding.

Figure 7

HIV-1 particle release detected upon expression of RFP-ALIXV-PRD wild type and the dimerization mutants.

(A) Expression of RFP-ALIXVmut1-PRD reverses the dominant negative effect observed for expression of wild type RFP-ALIXV-PRD. Lane 1, vector control; lane 2, wild type RFP-ALIXV-PRD; lane 3, RFP-ALIXVmut1-PRD. Detection of extracellular Gag levels (virions, left panel) and intracellular Gag levels (right panels) are shown.

(B) Expression of RFP-ALIXVmut2-PRD does not influence the dominant negative effect observed for expression of wild type RFP-ALIXV-PRD. Lane 1, vector control; lane 2, wild type RFP-ALIXV-PRD; lane 3, RFP-ALIXVmut2-PRD.

(C) Expression levels of RFP-ALIXV-PRD are shown for wild type (lane 2) and RFP-ALIXVmut1-PRD (lane 3) expression (lane 1, RFP vector expression control).