Figure 7.

Effects of preincubation with the reducing agent dithiothreitol (DTT) on SNP-induced ionic conductances. Oocytes expressing AQP1 wild type and double mutant D237A + K243C channels, and control oocytes were preincubated in ND96 saline with and without 10 mM DTT for 1 hour prior to electrophysiological recording of the response to SNP. Initial data are prior to SNP application; net conductances are (final)-(initial) for the SNP doses (mM) as indicated. An asterisk (*) indicates values that are significantly different (p < 0.006) from control; the pound sign (#) indicates values that are significantly different (p < 0.0001) from wild type AQP1 (unpaired two-tailed Student's t test) for comparisons within the same treatment group (with or without DTT). For other statistical comparisons, see details in text.