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Receptor-independent stimulation of amylin (top) and somatostatin (bottom) from cultured D-cells. Cells were stimulated with phorbol-12-myristate-13-acetate 100 nM (PMA), forskolin 10 μM (FSK) and A23187 1 μM for 2 hours. Results expressed as % of cell content of peptide released, mean ± SEM, ** P < 0.01 vs. control, * P < 0.05 vs. control.
