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. 2009 Apr 28;58(7):1672–1681. doi: 10.2337/db08-0183

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© 2009 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 4. — Real-time PCR of Col8a1 mRNA of MMCs in response to different cytokines. Wild-type MMCs were stimulated with 25 ng/ml EGF, bFGF, PDGF-BB, or TGF-β1 for 12, 24, and 48 h. After 12 h (A) strong Col8a1 expression was seen by PDGF-BB and bFGF and none by TGF-β1 and EGF. TGF-β1 was the strongest inductor of Col8a1 after 24 h followed by PDGF-BB, EGF, and bFGF (B). C: Stimulation for 48 h with TGF-β1 still had an effect on the Col8a1 expression, but none by bFGF, EGF, and PDGF-BB. Bars represent SE; *P < 0.05.