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. 2009 Apr 28;58(7):1672–1681. doi: 10.2337/db08-0183

FIG. 7.

FIG. 7.

Overexpression of Col8a1 increased proliferation of MMCs. Stimulation with glucose (A) or 50 ng/ml PDGF-BB (C) for 24 h leads to an increase in thymidine incorporation of Col8a1 MMCs compared with mock-transfected MMCs. Cell counts confirmed increased growth of Col8a1 MMCs compared with mock-transfected cells (stimulation with glucose [B] or PDGF-BB [D]). Bars represent SE. *P < 0.05; **P < 0.01. Western blots stained with the phosphorylation specific p42/p44 antibody revealed an induction of p42/p44 in Col8a1 MMCs, which was low in mock-transfected MMCs (E). CON, control; KO, knockout; WT, wild-type.