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. 2009 Apr 28;58(7):1641–1650. doi: 10.2337/db08-1365

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© 2009 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 8. — Decreased pSmad1/5/8 phosphorylation is evident in wild-type but not grem1^+/− diabetic kidney. A: Protein extracts from renal poles of control (C) and 27-week diabetic (D) wild-type (□) and grem1^+/− (■) mice were probed via Western blot using phospho-Smad1/5/8, total Smad1/5/8, and β-actin antibodies as described. n = 3 mice per group. B: Densitometry was performed using Scion Image software. pSmad1/5/8 intensities were normalized to total Smad1/5/8 levels and plotted as relative intensity. *P < 0.05 using Student's unpaired t test.