FIG. 5.

Leptin does not affect Ca²⁺ signals in the presence of wortmannin. A: In the presence of the PI3K inhibitor wortmannin (20 nmol/l), leptin was unable to affect the intracellular Ca²⁺ oscillations induced with 0.5 mmol/l glucose in αTC1-9 cells (n = 8). B: Average frequency of Ca²⁺ signals with 0.5 mmol/l glucose (control) and leptin, both in the presence of wortmannin. Data in B and C are shown as means ± SE. G, glucose; Lept, leptin; ns, nonsignificant.