FIG. 3.

NADPH oxidase is required for the insulin-induced hypothalamic ROS increase and food intake inhibition. Effect of DPI on insulin-induced ROS level change and food intake inhibition is shown. DPI (0.1 mmol/l) was intracerebroventricularly injected 30 min before insulin (0.4 μU). ROS levels were assessed in the hypothalamus 15 min after injection (n = 10 animals per group) (A). Food intake was assessed 12 h after food presentation (n = 5–10 animals per group) (B). Data are the means ± SE. *P < 0.05, **P < 0.01, ***P < 0.001 vs. vehicle- or insulin-injected group. DCF, dichlorofluorescein.