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. 2009 Apr 14;58(7):1499–1508. doi: 10.2337/db08-1571

FIG. 6.

FIG. 6.

High-fat–fed LH mice exhibit hepatic insulin resistance. A: Relative levels of PGC-1α mRNA isolated from livers of wild-type (WT) and LH mice fed either a regular chow or high-fat diet (HFD) for 16 weeks. Bars represent the means ± SE (n = 6) and are expressed relative to values for wild-type mice fed regular chow. B: Average weight of wild-type and LH mice on high-fat diet for 6 weeks. Data are the means ± SE (n = 10). C: Western blot of phosphorylated Akt (pAkt) and total Akt in hepatic protein extracts from high-fat diet–fed wild-type or LH mice after intravenous injection of PBS (−) or insulin (+). D and E: Circulating insulin (D) and hepatic mRNA expression (E) levels in high-fat diet–fed mice fasted overnight or fasted mice refed for 2 h. Data are the means ± SE expressed relative to wild-type fasted levels (n = 6–14). F: Hepatic mRNA levels in high-fat–fed wild-type and LH mice. Data are the means ± SE (n = 6), expressed relative to wild-type levels. *P < 0.05; **P < 0.01. Hsp90, 90-kDa heat shock protein.