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. Author manuscript; available in PMC: 2010 Mar 31.
Published in final edited form as: Neuroscience. 2009 Jan 19;159(3):1055–1069. doi: 10.1016/j.neuroscience.2009.01.023

Figure 2.

Figure 2

GFAP positive astrocytes are reduced in APP mice when NOS2 is genetically deleted and their contact with blood vessels is significantly reduced in mice with high vascular amyloid. Panels A-F show GFAP immunohistochemistry of astrocytes surrounding blood vessels in APPSw (A and B), APPSwDI (C and D) and APPSwDI/NOS2−/− (E and F) mice. Panels A, C and E show 400× magnification, scale bar = 6.25µm. Panels B, D and F show 600× magnification using differential interference contrast (DIC), scale bar = 4.2µm. In panels B, D and F the arrows indicate a visible blood vessel wall while the asterisk shows an example of a GFAP-positive process that was counted. Panel G shows average results of stereological counts of intact astrocyte processes contacting blood vessels ± SEM. Panel H shows average stereological quantification of astrocyte cell number ± SEM. ** indicates P<0.01 compared to wildtype and NOS2−/−.