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. Author manuscript; available in PMC: 2009 Jun 22.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Jul 20;361(3):745–750. doi: 10.1016/j.bbrc.2007.07.052

Figure 4. Direct interaction between splenocytes and MSCs induced the expression of IL-6 by MSCs.

Figure 4

(A) MSCs and splenocytes were cultured separately or together for 3 days and the amount of IL-6 in the supernatant measured using Bio-Plex analysis. (B) Splenocytes and MSCs were then harvested separately from either monoculture or the co-culture (MSCs are adherent) and expression of IL-6 mRNA was determined by real time PCR. Results shown as fold increase relative to β-actin. All values represent means ± SD of three separate samples. Data are representative of two independent experiments. SPL (MSC) = splenocytes from co-culture with MSCs; MSC (SPL) = MSCs from co-culture with splenocytes.