Table 1.
sEH transcription and expression and transfection efficiency in human cell lines
| Cell Line | Ref. | Origin | Disease | sEH specific activity (pmol/min/mg)a | Relative EPHX2 mRNA (EPHX2/GAPDH)a | Renilla luciferase activitya (RLU/1×104 cells)b |
|---|---|---|---|---|---|---|
| HepG2 | [44] | liver | carcinoma | 310±50 | 0.18±0.03 | 600±30 |
| HuH-7 | [41] | 940±40 | 0.99±0.20 | 130±30 | ||
|
| ||||||
| 293T | [39] | kidney | nonec | 210±10 | 0.54±0.08 | 98000±11000 |
| ACHN | [42] | adenocarcinoma | 31±15 | 0.38±0.04 | 30±20 | |
| SN12C | [43] | carcinoma | n.d.d | 0.08±0.02 | 240±60 | |
|
| ||||||
| Caco-2 | [38] | colon | adenocarcinoma | 120±10 | 0.33±0.02 | 780±70 |
| SW480 | [37] | 130±30 | 0.68±0.08 | 7000±700 | ||
|
| ||||||
| DU 145 | [36] | prostate | carcinoma | 440±10 | 0.39±0.03 | 9500±400 |
|
| ||||||
| HeLa | [40] | cervix | adenocarcinoma | 52±15 | 0.14±0.05 | 3400±200 |
a
The control pRL-CMV plasmid contains a luciferase gene from Renilla reniformis under the control of a CMV promoter.
b
Values are mean ± SD from three different experiments.
c
Human cell line, derived from embryo kidney, expressing SV40 large T antigen abundantly.
d
n.d. indicates not detectable under the condition of our assay.