Figure 2.

Restricted range of PF activation by a stimulating pipette. (A) Diagram of experimental arrangement. A stimulating pipette was used to activate PFs, which produced excitatory postsynaptic potentials (EPSCs) in Purkinje cells voltage clamped with a recording pipette. (B) Volume-rendered fluorescence image of a Purkinje cell, illustrating actual experimental arrangement. Box indicates area shown in C. (C) Positions of the PF stimulating pipette at the times the recordings shown in D were made. (D) PF-EPSCs evoked when the stimulating pipette was moved. (E) Relationship between PF current integrals and location of the stimulating pipette. Points represent the mean of six separate experiments and bars indicate the SEM.