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. Author manuscript; available in PMC: 2010 May 8.
Published in final edited form as: J Mol Biol. 2009 Mar 13;388(3):431–442. doi: 10.1016/j.jmb.2009.03.014

Table I.

Best Fit Parameters for RFC Mechanism (±PCNA)

Parameters PCNA No PCNA
kR-ATP_On ATP binding (μM−1 s−1) 100 100
kR-ATP_Off ATP release (s−1) 100 100
kR_Activation RFC activation (s−1) 4.64 ± 0.02 1.55 ± 0.01
kR_ATPase RFC ATPase (s−1) 0.046 ± 0.002 0.017 ± 0.0004
kR(A)_ATPase RFC(A) ATPase (s−1) 0.072 ± 0.0006 0.12 ± 0.001
kR(A)-DNA_On RFC(A)-DNA binding (μM−1 s−1) 50 50
kR(A)-DNA_Off RFC(A)-DNA release (s−1) 0.5 0.5
kR(A)DNA_ATPase RFC(A)-DNA ATPase (s−1) 53.9 ± 0.04 11.1 ± 0.1
kR-Pi_Off Pi release (s−1) 6.41 ± 0.003 13.53 ± 0.01
kR-DNA_Off DNA(PCNA) release (s−1) 1.7 ± 0.003 25.63 ± 2.7
NATP ATPase sites 3.11 ± 0.001 2.12 ± 0.0004
kPBP-Pi_On PBP-Pi binding (μM−1 s−1) 14 12
kPBP-Pi_Off PBP-Pi release (s−1) 1.2 1.2

Parameter values shown without confidence intervals were fixed during global data fitting.