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. Author manuscript; available in PMC: 2009 Jun 22.
Published in final edited form as: J Immunol Methods. 2007 May 24;324(1-2):48–62. doi: 10.1016/j.jim.2007.05.001

Figure 4. Birbeck granules can be visualized by immuno EM with L31.

Figure 4

(A) CHO cells stably transfected with mLangerin (CHO/mLangerin) were processed for electron microscopy and analyzed for the presence of Birbeck granules (middle and right panels, filled arrow). No BGs were detected in control CHO/Neo cells (left panel).

(B) CHO cells transfected with mLangerin (middle and right panels) were processed for electron microscopy and incubated with L31 (right panel) or the matching isotype control (middle panel) followed by 12 nm colloidal gold particles conjugated anti-rat mAbs (open arrow). CHO cells transfected with irrelevant cDNA as negative control (left panel, CHO/Neo) did not display membrane superimposition and were not stained with L31 mAb.