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. Author manuscript; available in PMC: 2009 Jun 22.
Published in final edited form as: J Immunol Methods. 2007 May 24;324(1-2):48–62. doi: 10.1016/j.jim.2007.05.001

Figure 6. Expression of Langerin in different mouse strains.

Figure 6

(A) The indicated organs from BALB/c mice were lysed and 30 μg were applied to 10 % SDS-PAGE, transferred onto PVDF membrane and immunoblotted using L31 mAb and anti-tubulin.

(B) Elevated expression levels of Langerin in different tissues of BALB/c (“B”) mouse are detected in comparison to C57BL/6 (“C”) mouse by Western blot analyses with L31 mAb and anti-tubulin as described in (A).

(C) F1 (BALB/c × C57BL/6) mice maintain the CD11chi Langerinlow population that is detected in BALB/c but not C57BL/6 mice. Peripheral LNs and spleen were treated with Collagenase D and the low density fractions from a 30 % BSA gradient were used to enrich DCs. After gating out autofluorescent cells by using the FL3 channel, large cells (defined by FSC versus SSC) were analyzed by flow cytometry for the expression of total Langerin after fixation and permeabilization (with L31), and surface CD11c and MHC II.