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. Author manuscript; available in PMC: 2009 Jun 22.
Published in final edited form as: J Immunol Methods. 2007 May 24;324(1-2):48–62. doi: 10.1016/j.jim.2007.05.001

Figure 7. Surface expression of mLangerin as detected with L31 mAb.

Figure 7

DCs from BALB/c mice were enriched by negative selection. In brief, spleen and skin draining LN cell suspensions were incubated with biotin-labeled anti-CD3, B220, CD19, DX5, Gr1 and Thy1.2 mAbs. The non-DCs were removed by the application of a magnetic field after the incubation with streptavidin-beads. Cells were stained with Alexa 647 labeled L31 mAb, or the corresponded isotype control, and CD11c FITC. For the total staining, the cells were fixed and permeabilized with cytofix/cytoperm buffer (BD Biosciences) following the manufacture’s instructions.