Table 1.
Differentially expressed genes in AP-2α low-expressing GN-11 clones
| Gene | Description | Accession number | FC |
| Cell cycle | |||
| Ccnd1 | cyclin D1 (Ccnd1), mRNA. | NM_007631 | -3.0 |
| Cetn3 | centrin 3 (Cetn3), mRNA. | NM_007684 | -2.7 |
| Cdc2a | cell division cycle 2 homolog A (S. pombe) | NM_007659 | -2.4 |
| Cdkn2a | cyclin-dependent kinase inhibitor 2A (Cdkn2a), mRNA. | NM_009877 | -2.4 |
| Skp2 | S-phase kinase-associated protein 2 (p45) | NM_013787 | -2.2 |
| Kras2 | Kirsten rat sarcoma oncogene 2, expressed (Kras2), mRNA. | NM_021284 | -2.1 |
| Pa2g4 | proliferation-associated 2G4 (Pa2g4), mRNA. | NM_011119 | 2.2 |
| Stmn1 | stathmin 1 (Stmn1), mRNA. | NM_019641 | 2.3 |
| Tgif | TG interacting factor (Tgif), mRNA. | NM_009372 | 2.4 |
| Anapc1 | anaphase promoting complex subunit 1 (Anapc1), mRNA. | NM_008569 | 2.5 |
| Atf5 | activating transcription factor 5 (Atf5), mRNA. | NM_030693 | 2.5 |
| Cdk5rap3 | CDK5 regulatory subunit associated protein 3 (Cdk5rap3), mRNA. | NM_030248 | 2.8 |
| Apoptosis | |||
| Tnfrsf12a | tumor necrosis factor receptor superfamily, member 12a (Tnfrsf12a), mRNA. | NM_013749 | -2.8 |
| Ngfrap1 | nerve growth factor receptor (TNFRSF16) associated protein 1 (Ngfrap1), mRNA. | NM_009750 | -2.7 |
| Ppm1f | protein phosphatase 1F (PP2C domain containing) (Ppm1f), mRNA. | NM_176833 | -2.7 |
| Gpx1 | glutathione peroxidase 1 (Gpx1), mRNA. | NM_008160 | -2.7 |
| Siva | Cd27 binding protein (Hindu God of destruction) (Siva), mRNA. | NM_013929 | -2.6 |
| Birc5 | baculoviral IAP repeat-containing 5 (Birc5), mRNA. | NM_009689 | -2.6 |
| Prkar1a | protein kinase, cAMP dependent regulatory, type I, alpha (Prkar1a), mRNA. | NM_021880 | -2.5 |
| Cycs | cytochrome c, somatic (Cycs), mRNA. | NM_007808 | -2.5 |
| Syvn1 | synovial apoptosis inhibitor 1, | NM_028769 | 2.5 |
| Sqstm1 | sequestosome 1 (Sqstm1), mRNA. | NM_011018 | 2.9 |
| Development | |||
| Emp1 | epithelial membrane protein 1 (Emp1), mRNA. | NM_010128 | -3.0 |
| Csrp2 | cysteine and glycine-rich protein 2 (Csrp2), mRNA. | NM_007792 | -2.6 |
| Crip2 | cysteine rich protein 2 (Crip2), mRNA. | NM_024223 | 2.5 |
| ECM/cell adhesion | |||
| Lox | lysyl oxidase (Lox), mRNA. | NM_010728 | -3.4 |
| Timp1 | tissue inhibitor of metalloproteinase 1 (Timp1), mRNA. | NM_011593 | -3.2 |
| Timp2 | tissue inhibitor of metalloproteinase 2 (Timp2), mRNA. | NM_011594 | -3.0 |
| Col6a1 | procollagen, type VI, alpha 1 (Col6a1), mRNA. | NM_009933 | -2.8 |
| Col5a1 | procollagen, type V, alpha 1 (Col5a1), mRNA. | NM_015734 | -2.5 |
| Mmp2 | matrix metalloproteinase 2 (Mmp2), mRNA. | NM_008610 | -2.0 |
| Ptprs | protein tyrosine phosphatase, receptor type, S (Ptprs), mRNA. | NM_011218 | 2.5 |
| Mfge8 | milk fat globule-EGF factor 8 protein (Mfge8), mRNA. | NM_008594 | 2.6 |
| Cell migration | |||
| Ryk | receptor-like tyrosine kinase (Ryk), mRNA. | NM_013649 | -2.7 |
| Rtn4 | reticulon 4 (Rtn4), transcript variant 5, mRNA. | NM_194054 | -2.7 |
| Axl | AXL receptor tyrosine kinase (Axl), mRNA. | NM_009465 | -2.6 |
| Wisp1 | WNT1 inducible signaling pathway protein 1 (Wisp1), mRNA. | NM_018865 | -2.5 |
| Ccl7 | chemokine (C-C motif) ligand 7 | NM_013654 | -2.4 |
| Cyb5 | cytochrome b-5 (Cyb5), mRNA | NM_025797 | -2.3 |
| Nup62 | nucleoporin 62 (Nup62), mRNA. | NM_053074 | 2.3 |
| Others | |||
| Anxa5 | annexin A5 (Anxa5), mRNA. | NM_009673 | -3.0 |
| Sumo1 | SMT3 suppressor of mif two 3 homolog 1 (yeast) | NM_009460 | -3.0 |
| Sms | spermine synthase (Sms), mRNA. | NM_009214 | -3.0 |
| Cenpa | centromere autoantigen A (Cenpa), mRNA. | NM_007681 | -2.9 |
| Plec1 | plectin 1 (Plec1), transcript variant 1, mRNA. | NM_011117 | -2.9 |
| Lmna | lamin A (Lmna), mRNA. | NM_019390 | -2.9 |
| Nedd8 | neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8), mRNA. | NM_008683 | -2.8 |
| Pfdn5 | prefoldin 5 (Pfdn5), mRNA. | NM_020031 | -2.7 |
| Rab2 | RAB2, member RAS oncogene family (Rab2), mRNA. | NM_021518 | -2.7 |
| Fscn1 | fascin homolog 1, actin bundling protein (Strongylocentrotus) purpuratus) (Fscn1), mRNA. | NM_007984 | -2.6 |
| Serpinb6a | serine (or cysteine) proteinase inhibitor, clade B, member 6a (Serpinb6a), mRNA. | NM_009254 | -2.6 |
| S100a1 | S100 calcium binding protein A1 (S100a1), mRNA. | NM_011309 | -2.5 |
| Timm8a | translocase of inner mitochondrial membrane 8 homolog a (yeast) | NM_013898 | -2.3 |
| Ppap2a | phosphatidic acid phosphatase 2a (Ppap2a), mRNA. | NM_008903 | -2.2 |
| Fez2 | fasciculation and elongation protein zeta 2 (zygin II) | NM_199448 | -2.0 |
| Ankrd1 | ankyrin repeat domain 1 (cardiac muscle) (Ankrd1), mRNA. | NM_013468 | 1.7 |
| Pole | polymerase (DNA directed), epsilon (Pole), mRNA. | NM_011132 | 1.9 |
| Naglu | alpha-N-acetylglucosaminidase (Sanfilippo disease IIIB) (Naglu), mRNA. | NM_013792 | 2.0 |
| Ruvbl2 | RuvB-like protein 2 (Ruvbl2), mRNA. | NM_011304 | 2.0 |
| Gig1 | glucocorticoid induced gene 1 (Gig1), mRNA. | NM_133218 | 2.1 |
| Soat1 | sterol O-acyltransferase 1 (Soat1), mRNA. | NM_009230 | 2.1 |
| Ris2 | retroviral integration site 2 (Ris2), mRNA. | NM_026014 | 2.8 |
| Ier3 | immediate early response 3 (Ier3), mRNA. | NM_133662 | 2.8 |
Microarray analysis (Illumina Ref-8 BeadChip) was performed on GN-11 pSUPER-empty and AP-2α-low-expressing (α4-c) clones in duplicate and 510 modulated genes were found (305 decreased, 205 increased, see Methods). A selection of the most interesting modulated genes is shown. FC = Fold change.