Figure 3.

EAAT3 is pentagonal in the plane of the plasma membrane. Fourteen representative EAAT3 particles are shown in the P face of the plasma membrane. In many particles, five “domains” (arrowheads), and a central feature (arrows) were observed. Below each image, the pentagonal structure of the same particle is emphasized by superimposing the outline of the EAAT3 complex, as well as the center-to-vertex connections within the pentagonal arrangement. The viewing angle for each particle was estimated from the height and position of the central feature in the pentameric complex (see text). Angles are reported with respect to the plane of the plasma membrane (considered to be the horizontal). (Scale bar = 10 nm.)