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Limited proteolytic digestion of TspGWI. Purified native TspGWI was subjected to proteolytic digestion on immobilised TPCK-trypsin. Lane M, protein marker (Sigma), bands marked: 205 kDa, myosin; 116 kDa, β-galactosidase; 97.4 kDa, phosporylase b; 67 kDa, bovine serum albumin; 45 kDa, ovoalbumin; 29 kDa, carbonic anhydrase; lane 1, proteolysis products. Out of 10 polypeptides obtained, the two subjected to N-terminal sequencing are marked with horizontal arrows.
