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Effect of divalent cations on the enzymatic activities of wt TspGWI and selected mutants of it.
| TspGWI variant | No divalent cation | Mg2+ | Ca2+ | Activity |
| WT | __ | +++ | __ | REase |
| WT | + | + | +++ | MTase |
| D58A | __ | __ | __ | REase |
| D58A | __ | +++ | +++ | MTase |
| N473A | __ | + | __ | REase |
| N473A | __ | __ | __ | MTase |
