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Methylation and restriction activities of TspGWI mutants.
| Mutation | REase activity | MTase activity |
| WT | +++ | +++ |
| D58A | __ | +++ |
| D58R | __ | +++ |
| E72A | __ | +++ |
| E72D | +++ | +++ |
| E72L | __ | +++ |
| E72N | __ | +++ |
| E72R | __ | +++ |
| E72S | __ | +++ |
| K74A | __ | +++ |
| K74G | __ | +++ |
| K74P | __ | +++ |
| K74R | __ | +++ |
| V356M | + | +++ |
| N473A | + | __ |
| V356M/N473A | __ | __ |
