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Double immunofluorescence staining for MMP-9 or TIMP-1 and actin in smooth muscle cells of the middle cerebral artery after MCAO. (A-C) Photographs demonstrating the localization of MMP-9, actin immunostaining, and their co-localization in smooth muscle cells (yellow fluorescence in the merged picture). (D-F) TIMP-1 immunostaining, actin immunostaining, and their co-localization in the smooth muscle cells (white arrows). Scale bar, 50 μm.
