Table 2.
Thermodynamic parameters (denaturant concentrations at transition midpoints and corresponding free energies extrapolated to zero denaturant concentration) for Val100Gly cpn10 denaturation by GuHCl and urea (pH 7, 20°C) at various total protein concentration.
| Denaturant | [protein] (μM) | Midpoint (M) | ΔGU(H2O) (kJ/mol oligomer) | ΔGU(H2O) average (kJ/mol oligomer) |
| GuHCla | 10 | 0.7 | 10 ± 1 | |
| 30 | 0.7 | 14 ± 1 | ||
| 50 | 0.7 | 11 ± 2 | 12 ± 2 | |
| Ureab | 30 | 2.25 | 197 ± 6 | |
| 50 | 2.70 | 195 ± 6 | ||
| 70 | 3.00 | 200 ± 6 | 197 ± 6 |
a. ΔGU(H2O) calculated using a 7-mer to 7-mer reaction mechanism. b. ΔGU(H2O) calculated using a 7-mer to 1-mer reaction mechanism.