Figure 3.

Gene ontology summary and co-expression profiles. a) Gene ontology summary of diapause and early vitellogenic regulation, featuring the highest over-represented Biological Processes annotations within each group. b) Gene ontology summary and co-expression clusters of oogenic biased expression. Boxes feature the highest over-represented Biological Processes and Molecular Function annotations within each expression profile (P1-P5). The total number of over-represented functional annotations for each cluster are provided [see Additional file 16, 17, 18, 19, 20]. Clusters were generated with Short Time-series Expression Miner (STEM, [56,57]). At each time-step genes were considered up or down regulated when compared to male controls. Expression profiles with a correlation of at least 0.8 were clustered together, generating five groups [see Additional file 21, 23]. Each cluster shown is over-represented at a P-value < 0.05 in permutation tests. Numbers in brackets represent the relative gene expression changes to which profiles were clustered at each point in the time series.