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CE-LIFP analysis of TMR-BP-ds90mer and its binding to E. coli UvrAB and B. caldotenax UvrAB, showing that the DNA wrapping after UvrB binding to the probe is directed by UvrA. In this experiment, the reaction solutions contained 10 nM TMR-BP-ds90mer, 20 nM UvrA, and 50 nM UvrB. The reactions of UvrA, UvrB, and TMR-BP-ds90mer were conducted at 37 °C.
