Fig. 3.

Analysis of promoter activities of the polymorphic E1-b 5′-flanking region. Luciferase-based promoter activities were analyzed by cloning the E1-b 5′-flanking DNA region (-2763/+6) for different haplotypes into the pGL3-basic vector. The data shown depict means and S.D. values derived from five separate experiments, each performed in duplicate. Inclusion of the 2xAlu element results in a statistically significant decreased promoter activity in A549, HepG2, and 293A cells (*, p < 0.05; **, p < 0.01).