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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Feb 12;1793(7):1165–1173. doi: 10.1016/j.bbamcr.2009.02.001

Figure 8. TGFBR2 dependent apoptosis is suppressed by CDKN1A/p21 siRNA.

Figure 8

A. siRNA for CDKN1A/p21 suppresses CDKN1A/p21 reporter luciferase activity in the V-400R2 cell line. A dose related increase in luciferase reporter activity in the siRNA control treated cell line is demonstrated. B. Apoptosis in V-400R2 was measured using the Cell Death ELISA (Roche) after 48 hours of treatment with TGF-β (10ng/ml). Treatment with the CDKN1A/p21 siRNA inhibits the TGFBR2 dependent induction of apoptosis, which is seen in the siRNA control treated cells. C. TGF-β mediated apoptosis is inhibited by SMAD7. Apoptosis was induced by TGF-β in the V-400R2 cell line after transfection with SMAD7. SMAD7 abrogates the increased apoptosis observed in the cells V-400R2 cells treated with increasing concentrations of RG-115819.