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Expression of recombinant TcKAPs in E. coli. The TcKAP4 (A) and TcKAP6 (B) were expressed in E. coli M15 strain following induction with 1 mM IPTG for 3 h. Immunoblotting assays of non-induced (1) and induced (2) bacterial extract using anti-polyhistidine antibody confirmed the expression of recombinant TcKAPs.
