Figure 4.

Effect of the Src kinase inhibitor PP2 on epileptiform burst frequency activity in the CA3 region of hippocampal induced either by perfusion with Mg²⁺-free medium or by application of 4-AP (50 μM). (A) The frequency of extracellularly recorded field bursts induced by Mg²⁺-free medium was significantly reduced (P < 0.01) in slices incubated with PP2 (10 μM). The burst frequency recovered significantly at 1 h of washout (P < 0.01 from slices treated with Mg²⁺-free/PP2; P > 0.05 from slices treated with Mg²⁺-free). (B) The mean frequency of extracellularly recorded field bursts was significantly reduced (P < 0.01) in slices incubated with 4-AP containing PP2 (10 μM). The burst frequency recovered partially but not significantly at 1 h of washout (P > 0.05 from slices treated with 4-AP/PP2; P < 0.05 from slices treated with 4-AP). Thus although epileptiform activities induced by Mg²⁺-free medium or by application of 4-AP were lower than the activity induced with Mg²⁺-free aCSF/4-AP/PP2, both were significantly reduced by PP2 treatment. **, Different (P < 0.01) from slices treated with Mg²⁺-free medium (A) or with 4-AP (B).