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. 2009 Apr 10;113(23):5757–5764. doi: 10.1182/blood-2009-01-201939

Figure 2.

Figure 2

Lack of lymphoid lineage priming in VCAM-1−RAG1− MPPs that are specified to the lymphoid lineage in vivo. Quantitative RT-PCR analysis of myeloid (A)- and lymphoid (B)-related gene expression in HSCs, MPPs, and CLPs. Relative expression level of each gene is normalized to GAPDH expression level. Expression level shown is the average value from triplicate samples from each cell population. The expression level of each gene in HSCs is arbitrarily set as 1. (C) Multiplex single-cell RT-PCR of lymphoid (IL-7Rα)- and myeloid (GM-CSFRα)-affiliated gene expression in MPP subsets. At least 50 HPRT+ wells were analyzed for the presence or absence of IL-7Rα and GM-CSFRα expression for each cell population.