Figure 3.

KRN7000 promotes proinflammatory cytokine secretion after allogeneic SCT. (A) Irradiated B6D2F₁ mice received whole spleen from G-CSF–mobilized B6 donors, followed by control diluent, C20:2, or KRN7000 IP on days +1 and +4. IFN-γ, TNF, IL-5, IL-4, and IL-10 were determined in the sera at various times thereafter by cytometric bead array (n = 8-12 per group and time point, combined from a minimum of 2 experiments, except for IL-5 and IL-4 at 48 hours and IL-10, where n = 3-4). *P < .05 vs control diluent; ^#P < .03 vs C20:2 and control diluent. (B) Irradiated B6 mice received whole spleen from G-CSF–mobilized BALB/c donors, followed by control diluent, C20:2, or KRN7000 IP on days +1 and +4. IFN-γ, TNF, IL-5, IL-4, and IL-10 were determined in the sera at various times thereafter by cytometric bead array (n = 8-12 per group and time point combined from a minimum of 2 experiments, except IL-5 and IL-4 at 48 hours and IL-10, where n = 4). *P < .05 vs control diluent; ^#P < .03 vs C20:2 and control diluent; ^##P < .001 vs C20:2 and control diluent.