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. Author manuscript; available in PMC: 2009 Jun 23.
Published in final edited form as: J Microsc. 2008 Jan;229(Pt 1):21–31. doi: 10.1111/j.1365-2818.2007.01870.x

Fig. 3.

Fig. 3

Relationship between mean optimum brightness-area-product threshold (BAPthr) and mean background intensity after adaptive histogram equalization (Ibahe). Each marker represents the mean across all frames in a scan. The solid line represents the relationship that was used to determine the BAPthr for a particular scan when Ibahe was calculated from the images. When Ibahe was less than 128, BAPthr was constant (2411), and otherwise it was equal to Ibahe x m1 + b1 (Eq. 4), where m1 and b1 are given in Table 3. The coefficients m1, b1, and the transition intensity changed after March 1, 1999 (data shown were from scans after this date).