TABLE 2.
Arachidonic acid incorporation coefficients (k*) in 5-HTT+/+, 5-HTT+/− and 5-HTT−/− mice at baseline and in response to DOI
| 5-HTT+/+ | 5-HTT+/− | 5-HTT−/− | Genotype × Drug Interaction |
Genotype effect | Drug effect | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Brain region | Saline (n = 5) |
DOI (n = 6) |
Saline (n = 5) |
DOI (n = 5) |
Saline (n = 5) |
DOI (n = 5) |
p and (F) values | p and (F) values | p and (F) values | |
| Prefrontal cortex layer I | 6.47 ± 0.44 | 8.84 ± 1.59* | 7.90 ± 1.36 | 6.56 ± 1.14 | 9.41 ± 1.44** | 4.89 ± 0.77** | < 0.001 (21.2) | 0.598 (0.53) | 0014 (7.05) | |
| Prefrontal cortex layer IV | 8.29 ± 0.91 | 10.4 ± 1.5 | 10.4 ± 1.0 | 8.70 ± 1.40 | 10.6 ± 1.9* | 6.32 ± 0.71** | < 0.001 (16.2) | 0.162 (1.96) | 0.009 (8.06) | |
| Primary olfactory cortex | 7.34 ± 0.76 | 9.19 ± 1.28* | 9.25 ± 0.78* | 7.93 ± 0.59 | 8.92 ± 1.19 | 5.50 ± 0.62*** | < 0.001 (21.4) | 0.007 (6.05) | 0.008 (8.30) | |
| Frontal cortex (10) | ||||||||||
| Layer I | 7.27 ± 1.12 | 8.14 ± 0.51 | 8.44 ± 0.69 | 7.87 ± 1.06 | 9.80 ± 1.12* | 7.01 ± 1.19* | 0.034 (3.89) | 0.565 (0.58) | 0.137 (2.36) | |
| Layer IV | 9.16 ± 1.30 | 10.4 ± 1.2 | 12.1 ± 0.5* | 9.00 ± 0.83* | 11.8 ± 1.8* | 8.10 ± 1.09* | 0.001 (9.17) | 0.476 (0.76) | 0.002 (12.17) | |
| Frontal cortex (8) | ||||||||||
| Layer I | 7.37 ± 1.00 | 10.3 ± 1.9** | 9.48 ± 1.07 | 8.49 ± 0.42 | 10.3 ± 1.5** | 6.17 ± 0.95*** | < 0.001 (20.62) | 0.388 (0.98) | 0.111 (2.73) | |
| Layer IV | 9.67 ± 1.08 | 13.0 ± 1.9** | 11.8 ± 0.8 | 9.10 ± 0.58* | 13.7 ± 1.9** | 6.48 ± 0.78*** | < 0.001 (23.44) | 0.073 (2.91) | 0.004 (10.34) | |
| Pyriform cortex | 6.11 ± 0.61 | 8.12 ± 1.34* | 7.87 ± 0.86 | 6.91 ± 1.48 | 8.49 ± 1.22** | 3.98 ± 0.44*** | < 0.001 (23.95) | 0.061 (3.14) | 0.006 (8.85) | |
| Anterior cingulate cortex | 9.74 ± 1.00 | 12.3 ± 1.4** | 11.9 ± 0.9* | 10.1 ± 1.2 | 12.7 ± 1.4** | 7.37 ± 1.22*** | < 0.001 (29.10) | 0.124 (2.27) | 0.002 (12.57) | |
| Motor cortex | ||||||||||
| Layer I | 6.42 ± 1.10 | 8.09 ± 0.52 | 8.82 ± 1.57* | 7.76 ± 0.68 | 9.92 ± 2.69** | 7.06 ± 0.91* | 0.006 (6.28) | 0.133 (2.20) | 0.169 (2.00) | |
| Layer II -III | 6.80 ± 0.97 | 9.07 ± 0.71* | 9.67 ± 1.31** | 8.76 ± 1.00 | 10.4 ± 2.0*** | 6.94 ± 0.65*** | < 0.001 (15.77) | 0.059 (3.17) | 0.107 (2.79) | |
| Layer IV | 8.96 ± 1.22 | 12.1 ± 1.3*** | 11.8 ± 0.8** | 10.4 ± 1.3 | 13.0 ± 1.3*** | 7.89 ± 0.73*** | < 0.001 (33.37) | 0.356 (1.078) | 0.013 (7.09) | |
| Layer V | 7.04 ± 0.49 | 9.94 ± 1.17*** | 10.2 ± 1.5*** | 8.80 ± 0.99 | 9.82 ± 1.35** | 6.34 ± 0.75*** | < 0.001 (23.35) | 0.023 (4.42) | 0.113 (2.70) | |
| Layer VI | 6.58 ± 0.53 | 9.59 ± 1.08*** | 9.62 ± 1.14*** | 8.94 ± 1.07 | 9.80 ± 1.08*** | 6.19 ± 1.10*** | < 0.001 (32.08) | 0.008 (5.86) | 0.219 (1.59) | |
| Somatosensory cortex | ||||||||||
| Layer I | 6.99 ± 0.65 | 10.0 ± 0.8*** | 9.38 ± 0.74** | 8.53 ± 0.97 | 10.4 ± 1.2*** | 8.70 ± 1.21* | < 0.001 (18.88) | 0.064 (3.08) | 0.606 (0.274) | |
| Layer II - III | 8.13 ± 0.85 | 11.2 ± 0.7*** | 11.1± 1.1*** | 8.81 ± 0.80** | 10.9 ± 1.3*** | 8.07 ± 1.01*** | < 0.001 (28.65) | 0.567 (0.58) | 0.065 (3.71) | |
| Layer IV | 9.66 ± 0.89 | 13.1 ± 1.9** | 12.2 ± 1.1* | 10.9 ± 0.8 | 12.9 ± 1.8** | 9.86 ± 0.97** | < 0.001 (16.24) | 0.937 (0.07) | 0.549 (0.37) | |
| Layer V | 9.01 ± 1.09 | 12.1 ± 1.7** | 12.0 ± 1.2** | 10.6 ± 1.3 | 11.7 ± 1.7* | 7.92 ± 1.00*** | < 0.001 (16.90) | 0.082 (2.77) | 0.151 (2.19) | |
| Layer VI | 9.19 ± 1.08 | 11.4 ± 0.9* | 11.4 ± 1.0* | 9.92 ± 0.89 | 10.6 ± 1.7 | 8.65 ± 1.02 | < 0.001 (10.56) | 0.138 (2.14) | 0.303 (1.11) | |
| Auditory cortex | ||||||||||
| Layer I | 7.62 ± 1.43 | 9.84 ± 1.11* | 11.3 ± 0.8*** | 8.56 ± 1.15** | 11.2 ± 1.0*** | 6.84 ± 1.01*** | < 0.001 (25.55) | 0.052 (3.34) | < 0.001 (17.21) | |
| Layer IV | 9.23 ± 1.43 | 12.1 ± 1.3** | 12.1 ± 0.9** | 9.76 ± 0.67* | 11.9 ± 1.6** | 7.40 ± 0.34*** | < 0.001 (30.22) | 0.046 (3.48) | 0.003 (10.43) | |
| Layer VI | 8.09 ± 1.21 | 10.0 ± 0.8** | 10.9 ± 0.4*** | 9.07 ± 0.68** | 9.98 ± 1.1** | 6.38 ± 0.25*** | < 0.001 (33.00) | < 0.001 (12.95) | < 0.001 (16.79) | |
| Visual cortex | ||||||||||
| Layer I | 7.17 ± 1.22 | 9.42 ± 1.04* | 9.99 ± 1.71** | 8.26 ± 1.06 | 10.0 ± 0.7** | 6.47 ± 0.31*** | < 0.001 (19.21) | 0.148 (2.07) | 0.017 (6.51) | |
| Layer IV | 8.64 ± 0.65 | 12.3 ± 2.4*** | 11.5 ± 1.1** | 9.04 ± 0.72* | 10.2 ± 1.1 | 7.40 ± 0.34* | < 0.001 (20.78) | 0.015 (5.01) | 0.275 (1.25) | |
| Layer VI | 8.31 ± 0.86 | 10.9 ± 1.9* | 11.6 ± 0.9** | 8.31 ± 0.51** | 10.7 ± 1.8* | 7.03 ± 0.30*** | < 0.001 (20.01) | 0.174 (1.88) | 0.005 (9.69) | |
| Preoptic area (LPO/MPO) | 6.46 ± 0.50 | 7.60 ± 2.07 | 7.89 ± 0.46 | 6.44 ± 0.57 | 6.95 ± 0.71 | 4.58 ± 0.57** | 0.002 (7.87) | 0.011 (5.45) | 0.027 (5.55) | |
| Suprachiasmatic nu | 6.42 ± 0.81 | 8.93 ± 1.85* | 7.44 ± 1.09 | 6.20 ± 1.02 | 7.56 ± 1.45 | 4.12 ± 0.54*** | < 0.001 (15.59) | 0.009 (5.77) | 0.117 (2.63) | |
| Globus pallidus | 5.83 ± 0.65 | 6.33 ± 0.95 | 7.08 ± 0.59 | 6.27 ± 0.54 | 6.77 ± 0.87 | 4.13 ± 0.51*** | < 0.001 (12.80) | 0.003 (7.42) | 0.001 (14.68) | |
| Bed nu stria terminalis | 6.63 ± 0.86 | 7.22 ± 1.21 | 7.09 ± 0.70 | 6.49 ± 1.05 | 7.58 ± 0.45 | 6.50 ± 0.42 | 0.088 (2.68) | 0.801 (0.22) | 0.252 (1.38) | |
| Olfactory tubercle | 8.67 ± 1.06 | 9.91 ± 1.11 | 10.7 ± 0.8* | 8.29 ± 0.94** | 11.1 ± 1.4** | 6.54 ± 0.43*** | < 0.001 (22.5) | 0.352 (1.09) | < 0.001 (28.29) | |
| Diagonal band Dorsal | 7.97 ± 0.37 | 9.00 ± 2.3 | 8.81 ± 0.57 | 7.07 ± 1.09 | 10.9 ± 1.3** | 5.01 ± 0.58*** | < 0.001 (19.32) | 0.551 (0.61) | < 0.001 (22.93) | |
| Ventral | 8.09 ± 0.93 | 8.49 ± 1.83 | 8.59 ± 1.06 | 6.38 ± 1.30* | 11.5 ± 0.7** | 4.23 ± 0.68*** | < 0.001 (22.97) | 0.320 (1.19) | < 0.001 (50.10) | |
| Amygdala | 6.78 ± 0.28 | 7.57 ± 0.79 | 8.70 ± 0.96* | 6.68 ± 1.28* | 8.32 ± 1.1 | 5.38 ± 0.89*** | < 0.001 (11.27) | 0.152 (2.03) | < 0.001 (16.86) | |
| Hippocampus | ||||||||||
| CA1 | 5.91 ± 0.44 | 6.89 ± 0.62* | 8.23 ± 1.10** | 6.31 ± 0.37** | 7.17 ± 0.97 | 5.49 ± 0.27* | 0.001 (9.41) | 0.036 (3.82) | 0.009 (8.06) | |
| CA2 | 6.25 ± 0.39 | 7.27 ± 0.94 | 8.14 ± 0.42*** | 6.83 ± 0.38* | 7.72 ± 0.43* | 5.39 ± 1.06*** | < 0.001 (16.75) | 0.013 (5.18) | 0.002 (12.70) | |
| CA3 | 6.26 ± 0.70 | 7.57 ± 0.56 | 8.15 ± 0.53*** | 7.00 ± 0.70* | 8.29 ± 0.91** | 4.66 ± 0.50*** | < 0.001 (36.45) | 0.004 (7.00) | < 0.001 (23.38) | |
| Dentate gyrus | 7.01 ± 0.70 | 7.83 ± 0.85 | 8.84 ± 0.65** | 7.65 ± 0.64 | 8.71 ± 0.83** | 5.56 ± 0.62*** | < 0.001 (19.47) | 0.006 (6.29) | < 0.001 (20.09) | |
| SLM | 9.27 ± 1.12 | 11.0 ± 1.0 | 11.9 ± 1.2* | 9.74 ± 0.47 | 11.3 ± 1.1 | 7.96 ± 1.46** | 0.001 (9.97) | 0.171 (1.90) | 0.021 (6.12) | |
| Accumbens nu | 6.95 ± 0.69 | 7.72 ± 1.09 | 9.18 ± 0.62** | 7.89 ± 0.92 | 8.73 ± 0.79* | 5.84 ± 0.66*** | < 0.001 (13.07) | 0.003 (7.53) | 0.001 (14.57) | |
| Caudate putamen | ||||||||||
| Dorsal | 7.45 ± 0.91 | 8.66 ± 0.95 | 9.32 ± 1.49* | 7.55 ± 0.30* | 10.2 ± 0.8** | 5.07 ± 0.58*** | < 0.001 (31.28) | 0.161 (1.96) | < 0.001 (33.00) | |
| Ventral | 7.65 ± 0.32 | 9.39 ± 0.91** | 9.21 ± 0.74* | 7.53 ± 0.36* | 11.4 ± 1.4*** | 5.55 ± 0.64*** | < 0.001 (32.41) | 0.951 (0.05) | < 0.001 (24.79) | |
| Lateral | 7.34 ± 0.46 | 8.66 ± 0.75 | 9.67 ± 1.27* | 8.12 ± 0.73 | 11.8 ± 1.9*** | 5.76 ± 0.64*** | < 0.001 (25.09) | 0.192 (1.76) | < 0.001 (23.54) | |
| Medial | 7.28 ± 0.45 | 7.98 ± 0.94 | 9.26 ± 0.88* | 7.90 ± 0.52 | 10.9 ± 1.9*** | 5.24 ± 0.61*** | < 0.001 (26.61) | 0.124 (2.28) | < 0.001 (33.34) | |
| Septal nucleus lateral | 6.46 ± 0.74 | 6.30 ± 0.77 | 7.58 ± 0.74 | 6.19 ± 0.42* | 8.48 ± 0.67*** | 4.69 ± 0.61*** | < 0.001 (19.33) | 0.241 (1.51) | < 0.001 (54.26) | |
| Septal nucleus medial | 8.33 ± 1.01 | 9.22 ± 1.86 | 8.68 ± 0.62 | 7.26 ± 0.72 | 10.9 ± 1.1** | 4.86 ± 0.65*** | < 0.001 (25.32) | 0.146 (2.08) | < 0.001 (29.12) | |
| Diencephalon | ||||||||||
| Habenular nu lateral | 14.0 ± 1.6 | 16.5 ± 2.3 | 15.3 ± 1.7 | 13.8 ± 0.9 | 17.7 ± 2.0** | 9.03 ± 0.91*** | < 0.001 (29.60) | 0.050 (3.38) | < 0.001 (18.81) | |
| Habenular nu medial | 13.2 ± 2.3 | 16.8 ± 2.1 | 18.1 ± 4.8 | 13.7 ± 0.7 | 17.3 ± 1.7** | 8.41 ± 0.37*** | < 0.001 (17.49) | 0.032 (3.98) | 0.001 (13.54) | |
| Lateral geniculate nu | 10.7 ± 1.1 | 13.5 ± 1.0*** | 13.4 ± 1.1** | 10.5 ± 0.8*** | 14.6 ± 1.3*** | 8.13 ± 0.63*** | < 0.001 (55.61) | 0.247 (1.48) | < 0.001 (35.7) | |
| Medial geniculate nu. | 11.0 ± 1.3 | 14.4 ± 0.9*** | 14.4 ± 1.1*** | 11.5 ± 0.7** | 15.3 ± 0.9*** | 10.5 ± 1.8*** | < 0.001 (31.85) | 0.333 (1.15) | 0.017 (6.55) | |
| Thalamus | ||||||||||
| Ventroposterior lateral nu | 10.9 ± 0.9 | 12.7 ± 1.9 | 12.2 ± 1.00 | 9.32 ± 1.16* | 13.3 ± 1.0* | 7.31 ± 1.04*** | < 0.001 (24.22) | 0.034 (3.89) | < 0.001 ((27.53) | |
| Ventroposterior medial nu | 10.1 ± 1.5 | 12.6 ± 1.9 | 12.9 ± 1.5 | 9.62 ± 0.96* | 13.1 ± 1.4* | 7.79 ± 1.18*** | < 0.001 (20.57) | 0.303 (1.25) | 0.001 (14.76) | |
| Paratenial nu | 8.70 ± 1.38 | 10.5 ± 1.1 | 11.5 ± 1.3 | 9.21 ± 0.97 | 12.2 ± 1.9 | 6.73 ± 0.76** | < 0.001 (21.40) | 0.257 (1.44) | < 0.001 (19.40) | |
| Anteroventral nu | 12.8 ± 1.3 | 14.4 ± 1.5 | 16.8 ± 2.5* | 12.4 ± 0.7* | 17.7 ± 2.1* | 10.4 ± 0.5*** | 0.002 (8.28) | 0.786 (0.24) | 0.001 (14.30) | |
| Anteromedial nu | 9.51 ± 1.68 | 10.9 ± 1.1 | 13.2 ± 1.5** | 10.1 ± 1.1* | 13.4 ± 2.5** | 7.23 ± 1.08*** | < 0.001 (15.27) | 0.072 (2.93) | < 0.001 (21.79) | |
| Reticular nu | 9.98 ± 1.23 | 11.6 ± 1.9 | 10.7 ± 1.7 | 10.1 ± 0.7 | 12.7 ± 2.0* | 7.35 ± 0.89*** | < 0.001 (14.55) | 0.521 (0.67) | 0.014 (6.94) | |
| Paraventricular nu | 9.25 ± 1.39 | 10.6 ± 1.0 | 11.5 ± 1.3* | 9.62 ± 0.78 | 12.2 ± 1.9** | 6.73 ± 0.76*** | < 0.001 (19.97) | 0.167 (1.93) | < 0.001 (20.51) | |
| Parafascicular nu | 9.46 ± 1.33 | 11.3 ± 1.3 | 10.8 ± 0.8 | 10.1 ± 1.5 | 12.5 ± 2.7** | 6.17 ± 0.94*** | < 0.001 (19.25) | 0.194 (1.75) | 0.005 (9.66) | |
| Subthalamic nu | 10.4 ± 1.1 | 13.1 ± 1.0* | 12.6 ± 1.1 | 12.2 ± 1.2 | 14.5 ± 2.3*** | 12.4 ± 0.8 | 0.004 (7.06) | 0.044 (3.56) | 0.856 (0.034) | |
| Hypothalamus | ||||||||||
| Supraoptic nu | 7.04 ± 0.67 | 7.24 ± 0.87 | 8.89 ± 0.44** | 7.37 ± 0.46* | 7.75 ± 0.95 | 4.23 ± 0.68*** | < 0.001 (17.76) | < 0.001 (22.63) | < 0.001 (39.58) | |
| Lateral | 6.34 ± 0.21 | 7.23 ± 1.05 | 7.95 ± 0.99 | 6.37 ± 0.35 | 8.19 ± 1.88* | 4.30 ± 0.57*** | < 0.001 (14.64) | 0.145 (2.09) | < 0.001 (17.64) | |
| Anterior | 6.42 ± 0.39 | 7.44 ± 1.27 | 7.96 ± 0.87 | 6.29 ± 0.49 | 9.21 ± 2.28** | 4.83 ± 0.56*** | < 0.001 (13.73) | 0.930 (0.072) | 0.001 (15.62) | |
| Periventricular | 5.20 ± 0.36 | 6.48 ± 1.20 | 7.01 ± 0.65 | 5.05 ± 0.64* | 7.58 ± 1.90** | 4.01 ± 0.47*** | < 0.001 (15.25) | 0.863 (0.148) | 0.001 (14.75) | |
| Arcuate | 6.41 ± 0.90 | 7.77 ± 1.55 | 4.34 ± 1.03 | 6.15 ± 0.29 | 8.01 ± 1.34 | 4.88 ± 0.74*** | < 0.001 (11.41) | 0.406 (0.94) | 0.017 (6.48) | |
| Ventromedial | 6.64 ± 0.82 | 8.04 ± 1.45 | 7.84 ± 1.15 | 6.35 ± 0.28 | 7.98 ± 1.50 | 4.57 ± 0.69*** | < 0.001 (12.94) | 0.086 (2.71) | 0.006 (8.89) | |
| Posterior | 8.91 ± 1.88 | 11.7 ± 1.8 | 9.38 ± 0.56 | 8.07 ± 0.68 | 17.7 ± 3.3*** | 6.74 ± 1.02*** | < 0.001 (39.83) | 0.001 (9.66) | < 0.001 (24.29) | |
| Mammillary nu | 6.45 ± 0.82 | 8.41 ± 1.61 | 9.25 ± 0.78** | 8.15 ± 0.55** | 9.96 ± 1.71*** | 7.39 ± 1.09** | < 0.001 (14.37) | 0.063 (3.09) | 0.008 (8.22) | |
| Mesencephalon | ||||||||||
| Interpeduncular nu | 14.4 ± 2.6 | 23.1 ± 3.2*** | 19.5 ± 3.6 | 15.7 ± 1.1 | 23.6 ± 4.2*** | 13.7 ± 2.2*** | < 0.001 (25.93) | 0.658 (0.43) | 0.137 (2.37) | |
| Substantia nigra | 7.01 ± 0.98 | 9.75 ± 1.20** | 10.8 ± 1.0*** | 8.14 ± 1.06** | 9.68 ± 0.62** | 7.42 ± 1.47* | < 0.001 (20.06) | 0.074 (2.90) | 0.077 (3.39) | |
| Pretectal area | 9.49 ± 1.14 | 12.7 ± 2.5* | 12.1 ± 1.5 | 10.2 ± 1.4 | 13.0 ± 1.9* | 9.81 ± 1.36* | 0.001 (10.01) | 0.918 (0.09) | 0.344 (0.93) | |
| Grey layer Sup colliculus | 9.00 ± 1.56 | 10.5 ± 1.2 | 11.1 ± 0.7 | 9.52 ± 0.76 | 11.1 ± 1.8 | 7.37 ± 1.17*** | < 0.001 (11.10) | 0.181 (1.83) | 0.010 (7.72) | |
| Superior colliculus | 9.70 ± 1.16 | 13.0 ± 2.2* | 14.0 ± 2.5** | 9.68 ± 0.75** | 13.4 ± 1.9* | 8.09 ± 1.23*** | < 0.001 (19.16) | 0.380 (1.06) | 0.003 (11.12) | |
| Inferior colliculus | 14.7 ± 1.3 | 18.0 ± 0.9 | 18.5 ± 2.2 | 15.3 ± 1.7 | 20.5 ± 3.7** | 15.6 ± 2.1* | 0.001 (9.53) | 0.228 (1.57) | 0.063 (3.78) | |
| Median raphe nu | 7.70 ± 1.12 | 10.3 ± 0.9* | 11.3 ± 0.9*** | 11.0 ± 1.5 | 11.3 ± 0.9*** | 11.4 ± 1.5 | 0.016 (4.89) | < 0.001 (13.97) | 0.064 (3.25) | |
| Dorsal raphe nu | 8.56 ± 1.18 | 11.2 ± 1.5 | 10.9 ± 0.8 | 11.1 ± 1.1 | 11.4 ± 1.5 | 10.6 ± 0.9 | 0.173 ((1.89) | 0.585 (0.55) | 0.556 (0.36) | |
| Pedunculopontine tegmental nu | 7.88 ± 0.87 | 9.94 ± 1.48* | 9.56 ± 0.64 | 8.62 ± 0.84 | 9.56 ± 0.64** | 8.89 ± 0.362 | 0.002 (8.49) | 0.737 (0.31) | 0.655 (0.21) | |
| Rhombencephalon | ||||||||||
| Flocculus | 9.46 ± 1.02 | 10.8 ± 0.8 | 10.4 ± 1.1 | 10.3 ± 0.6 | 12.2 ± 1.3** | 8.98 ± 1.43*** | < 0.001 (12.24) | 0.585 (0.55) | 0.099 (2.94) | |
| Cerebellar gray matter | 7.93 ± 0.58 | 10.1 ± 1.2 | 11.4 ± 1.2** | 8.79 ± 0.94* | 12.5 ± 1.5*** | 7.95 ± 1.74*** | < 0.001 (19.67) | 0.062 (3.11) | 0.001 (13.82) | |
| Molecular layer cerebellar gray | 11.2 ± 1.2 | 15.9 ± 1.7** | 14.2 ± 1.8 | 13.0 ± 1.2 | 16.8 ± 2.4*** | 12.3 ± 2.4** | < 0.001 (16.33) | 0.407 (0.93) | 0.602 (0.28) | |
| Raphe magnus nu | 8.12 ± 0.84 | 11.3 ± 1.3* | 11.3 ± 2.1* | 10.5 ± 1.0 | 14.1 ± 2.8*** | 11.2 ± 2.0 | 0.003 (7.66) | 0.004 (6.97) | 0.794 (0.07) | |
| Raphe pallidus nu | 8.08 ± 1.7 | 11.5 ± 1.6* | 10.4 ± 1.0 | 8.96 ± 1.23 | 12.3 ± 3.7* | 8.1 ± 1.0* | 0.001 (10.26) | 0.813 (0.21) | 0.297 (1.13) | |
| Locus coeruleus | 9.09 ± 0.60 | 12.5 ± 1.9* | 11.7 ± 1.3 | 12.6 ± 1.9 | 14.6 ± 2.3*** | 13.8 ± 1.8 | 0.032 (3.98) | 0.001 (9.67) | 0.077 (3.39) | |
| Cochlear nu | 9.92 ± 1.52 | 11.9 ± 1.8 | 11.8 ± 0.5 | 11.7 ± 1.8 | 14.9 ± 1.0 | 12.9 ± 1.3 | 0.012 (5.26) | < 0.001 (12.28) | 0.945 (0.01) | |
| Spinal trigeminal nu, interpolar | 9.68 ± 1.22 | 12.6 ± 1.5 | 13.8 ± 2.1** | 9.53 ± 0.62** | 12.8 ± 1.8 | 9.45 ± 2.32* | < 0.001 (13.20) | 0.782 (0.25) | 0.011 (7.63) | |
| Superior olive | 7.12 ± 0.52 | 11.6 ± 3.1* | 11.9 ± 0.9* | 9.77 ± 1.31 | 11.3 ± 2.8* | 8.86 ± 2.9*** | < 0.001 (12.71) | < 0.001 (18.41) | < 0.001 (17.13) | |
| Medial vestibular nu | 9.84 ± 0.85 | 16.2 ± 3.2*** | 13.1 ± 2.1 | 10.5 ± 0.9 | 14.8 ± 2.6** | 7.60 ± 2.30*** | < 0.001 (25.62) | 0.165 (1.94) | 0.171 (1.98) | |
| White matter | ||||||||||
| Corpus callosum | 5.34 ± 0.60 | 6.06 ± 0.58 | 6.28 ± 0.77 | 5.25 ± 0.40 | 5.39 ± 0.26 | 3.85 ± 0.43*** | < 0.001 (13.01) | < 0.001 (14.52) | 0.004 (10.22) | |
| Zona incerta | 7.55 ± 0.63 | 9.04 ± 1.91 | 8.36 ± 1.07 | 7.84 ± 0.35 | 11.0 ± 1.9** | 7.95 ± 0.75*** | < 0.001 (23.01) | 0.829 (0.190) | 0.001 (12.97) | |
| Internal capsule | 5.09 ± 0.36 | 6.05 ± 1.08* | 5.83 ± 0.57 | 5.32 ± 0.48 | 6.11 ± 0.89* | 3.72 ± 0.88*** | < 0.001 (12.27) | 0.110 (2.42) | 0.028 (5.45) | |
| Cerebellar white matter | 5.66 ± 0.50 | 5.75 ± 0.64 | 5.95 ± 0.39 | 5.91 ± 0.33 | 7.45 ± 1.35** | 3.87 ± 0.65*** | < 0.001 (21.17) | 0.662 (0.42) | < 0.001 (20.41) | |
| Non-blood-brain barrier regions | ||||||||||
| Subfornical organ | 7.41 ± 1.16 | 10.7 ± 1.7* | 8.93 ± 0.81 | 8.59 ± 0.55 | 12.7 ± 3.5*** | 7.92 ± 0.89** | < 0.001 (14.10) | 0.131 (2.21) | 0.352 (0.90) | |
| Median eminence | 5.84 ± 0.78 | 8.17 ± 1.19* | 7.08 ± 0.87 | 5.09 ± 0.90* | 9.11 ± 1.47*** | 4.41 ± 0.70*** | < 0.001 (31.53) | 0.127 (2.25) | 0.001 (15.60) | |
| Choroid plexus (third ventricle) | 28.5 ± 3.2 | 31.0 ± 3.7 | 28.4 ± 6.4 | 29.3 ± 1.7 | 35.5 ± 9.5 | 24.1 ± 3.1** | 0.011 (5.47) | 0.893 (0.11) | 0.174 (1.96) | |
Abbreviations: nu, nucleus; SLM, stratum lacunosum-moleculae of hippocampus
k* = (ml/s/g) × 10−4, DOI: 1.5 mg/kg s.c. Each value is a mean ± S.D.
In cases of statistically significant genotype 5-HTT × drug interaction, Bonferroni' post-test were realized.
*
p < 0.05
**
p < 0.01
***
p < 0.001
5-HTT+/+ plus DOI vs 5-HTT+/+ saline; 5-HTT+/− saline vs 5-HTT+/+ saline, 5-HTT+/− plus DOI vs 5-HTT+/− saline, 5-HTT−/− saline vs 5-HTT+/+ saline, 5-HTT−/− plus DOI vs 5-HTT−/− saline