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. 2009 Apr 20;185(2):251–264. doi: 10.1083/jcb.200810130

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© 2009 Cui et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Faithful segregation of STB reporter plasmids is dependent on the Kip1p motor protein. (A) Segregation data for a fluorescence-tagged multicopy reporter plasmid in [cir⁺] strains were averaged from three separate experiments (≥100 cells per experiment). Error bars represent standard deviations from the mean. (B) The types of segregations for the indicated strains, assayed separately from A, were: equal (I), unequal (II), and failed segregation (III). Within II and III, segregations favoring the mother or daughter were not binned into distinct groups. Bar, 2 µm. (C) The scheme for assaying the segregation of a single-copy reporter plasmid is illustrated. The host strain, arrested in G1 using α factor, was further conditioned in glucose or galactose in the presence of the pheromone before being released into the cell cycle in glucose (CEN active) or galactose (CEN inactive; STB active). (D) The data shown are for cells released into the cell cycle in galactose. Bar, 2 µm. Results for cells released into glucose are given in Fig. S2. Individual values in B and D were derived by scoring a minimum of 100 cells.