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. 2009 Apr 20;185(2):203–211. doi: 10.1083/jcb.200809167

Figure 1.

Figure 1.

c-Myc induces SIRT1 expression. (a) Control vector or constructs encoding Flag–c-Myc were transfected into 293T cells. 48 h later, cell lysates were examined by Western blotting. (b) HeLa cells were transfected with control (ctrl) shRNA or c-Myc shRNA. 72 h later, proteins were examined by Western blotting. (c) HO15 (c-Myc−/−), TGR (c-Myc+/+), and Myc3 (HO15 cells reconstituted with c-Myc) were lysed, and the cell lysates were subjected to Western blotting. (d) mRNA from HO15 and Myc3 were subjected to QRT-PCR. Values represent the relative induction of SIRT1 mRNA normalized to GAPDH. Data represent the mean of three determinations ± SEM. **, P < 0.01 by two-tailed Student's t test. (e) ChIP assays were performed using anti–c-Myc antibody. The TERT and nucleolin promoter primers were used as a positive control, and SIRT1 intron 7 primers were used as a negative control.