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. 2009 Apr 20;185(2):203–211. doi: 10.1083/jcb.200809167

Figure 4.

Figure 4.

SIRT1 inhibits Myc target gene expression through c-Myc. (a) 293T cells were transfected with the indicated constructs. 48 h later, cells were lysed, and cell lysates were blotted with the indicated antibodies. (b) Cell lysates from SIRT1+/+ or SIRT1−/− MEFs were blotted with the indicated antibodies. (c) HO15 and Myc3 cells were infected with retrovirus control or retrovirus encoding SIRT1 and SIRT1-H363Y. 48 h later, cells were lysed, and cell lysates were blotted with the indicated antibodies. (d) Cell lysates from SIRT1+/+ and SIRT1−/− were blotted with the indicated antibodies. (e) HeLa cells were transfected with control (ctrl) or SIRT1 siRNA. 72 h later, cells were lysed, and cell lysates were blotted with the indicated antibodies. (f) 293T cells were transfected with the indicated constructs. 48 h later, cell lysates were blotted with the indicated antibodies. (g) SIRT1+/+ and SIRT1−/− were lysed, and mRNA were extracted and subjected to QRT-PCR. Values represent the relative induction normalized to GAPDH. Data represent the mean of three determinations ± SEM. **, P < 0.01 by two-tailed Student's t test. (h) HO15 and Myc3 cells were infected with retrovirus control or retrovirus encoding SIRT1. 48 h later, cells were lysed, and cell lysates were blotted with the indicated antibodies.