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Ub and p62 accumulate in α- and β′-COP–depleted 293/GFP-LC3 cells. (A) 293/GFP-LC3 cells were treated with control or β′-COP siRNA for 48 h, then fixed and labeled with anti-p62 and anti-ubiquitin antibodies for colocalization with GFP-LC3. (B) HEK293 cells treated as in A and labeled with anti-ubiquitin and anti-p62 antibodies. (C) Cryosections from β′-COP–depleted cells were labeled with anti-Ub antibodies followed by 10-nm protein A–gold. AV, autophagosomes.
