Figure 7.

GFP-LC3 AVs are LAMP2 positive. (A) 293/GFP-LC3 cells were incubated with control or β′- or α-COP siRNA for 48 h, fixed and labeled with anti-LAMP2 antibodies, followed by Alexa 555 anti–mouse antibodies. After COPI depletion many GFP-LC3–positive AVs are positive for LAMP2. Asterisk indicates cell enlarged in inset. (B) Cryosections of β′-COP-depleted cells labeled with anti-GFP (left) or anti-GFP and anti-LAMP2 (right) followed by protein A–gold as indicated. AV, autophagosomes, M, mitochondria.