Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Apr 20;185(2):291–303. doi: 10.1083/jcb.200811105

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Manzl et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — The PIDDosome is not required for the formation of high molecular weight complexes containing caspase-2. (A) Lysates from wild-type (wt), raidd^−/−, and pidd^−/− SV40-immortalized MEFs were incubated at 4°C or 37°C for 1 h and subjected to size-exclusion chromatography, and fractions were precipitated, separated by SDS-PAGE, and immunoblotted with an anti–caspase-2 antibody (Ab). (B) Fractions 3–5, containing complexes ≥600 kD, and fractions 17–19, containing proteins of a molecular weight range of ∼75–40 kD, of all indicated genotypes were analyzed for the relative caspase-2 expression level. Ponceau staining of the membranes was used to compare protein loading (bottom).