Table I.

Summary of stimuli and final concentrations used to induce cell death

Stimulus	Primary thymocytes	Splenic B cells	Splenic T cells	Bone marrow granulocytes	SV40-immortalized MEFs	Primary MEFs
Etoposide	1 µg/ml	1 µg/ml	1 µg/ml	1–10 µg/ml	10 – 50 µg/ml	10–50 µg/ml
Doxorubicin	400 ng/ml	400 ng/ml	400 ng/ml	400 ng/ml	ND	ND
Cisplatin	25–100 µM	50 µM	50 µM	50 µM	20 µM	ND
Staurosporine	100 and 500 nM	100 nM	100 nM	100 and 500 nM	50 nM	ND
Ionomycin	1 µg/ml	ND	ND	ND	ND	ND
PMA	10 ng/ml	ND	ND	ND	ND	ND
Velcade	50 ng/ml	50 ng/ml	50 ng/ml	ND	ND	ND
Trichostatin A	50 nM	50 nM	50 nM	ND	ND	ND
Dexamethasone	10−7 M	10−7 M	10−7 M	ND	ND	ND
TNF-α	10 ng/ml	10 ng/ml	10 ng/ml	10 ng/ml	30 ng/ml	ND
Cycloheximide	5 µg/ml	5 µg/ml	5 µg/ml	5 µg/ml	5 µg/ml	ND
Super Fas ligand	10 ng/ml	10 ng/ml	10 ng/ml	10 ng/ml	ND	ND
Cytochalasin D	2 µM	ND	ND	ND	20 µM	20 µM
Taxol	500 µM	ND	ND	ND	100 µM	100 µM
Vincristine	ND	ND	ND	ND	ND	50 µg/ml
Tunicamycin	0.1–5 µg/ml	1 µg/ml	1 µg/ml	ND	1.2 µg/ml	ND
Brefeldin A	1.25 and 2.5 µg/ml	ND	ND	ND	1 µg/ml	ND
Thapsigargin	0.05–0.1 µg/ml	ND	ND	ND	50 µg/ml	50 µg/ml