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. 2009 Apr 20;185(2):341–355. doi: 10.1083/jcb.200807155

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© 2009 Fejtova et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Mapping of three independently functional DLC-binding sites in Bassoon. (A) Bassoon constructs including the amino acid substitutions introduced at interaction sites I, II, and III and their relative lengths; bordering amino acid numbers refer to rat Bassoon. (B) Summary of binding assays in yeast (yeast two-hybrid [Y2H]) and in corecruitment assay by mito-targeting in COS-7 cells (Mito). (C) Molecular-binding activities (MBA) of purified Bsn fragments to DLC1 and DLC2 in surface plasmon resonance assays. Error bars indicate SEM. **, P < 0.01. (D) Recombinant proteins used for the binding assays are shown on a Coomassie-stained SDS gel. Molecular mass is indicated in kilodaltons.