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. 2009 Apr 20;185(2):341–355. doi: 10.1083/jcb.200807155

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© 2009 Fejtova et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Bassoon can serve as a cargo adapter for retrograde transport in COS-7 cells. (A–E) Cells were transfected with mito-targeting constructs and fixed 18 h later. The position of the MTOC is visualized by staining with anti–α-tubulin antibody in A2–D2; actin is visualized with Alexa Fluor 568–coupled phalloidin (E2). In cells expressing mito-targeted fragment Bsn9 containing the DLC-binding sites II and III, mitochondria accumulate at the MTOC. Mitochondria are distributed throughout the cytoplasm in cells expressing mito-targeted EGFP (row A), fragment Bsn9^II,III with mutated DLC-binding sites (row C), or in cells expressing mito-targeted Bsn9 after treatment with nocodazole for 2 h before fixation (row D). Depolymerization of actin filaments with cytochalasin D (2 h before fixation) does not prevent the effect of Bsn9 (E1–E3). Bar, 20 µm.